Mycoplasma Diagnostics Testing Have Revolutionized With the Advent of Real-Time PCR Technology
The tests for Mycoplasma infections are based on the culture of bacterial cells. The culture is performed on a sample of urine, urethra, and vaginal secretions. Positive cultures are a sign of a Mycoplasma infection, yet, it may not mean a definitive diagnosis. Mycoplasmas are a severe problem for cattle and can have significant economic and welfare impacts. Thus, it is important to perform Mycoplasma diagnostics promptly to prevent outbreaks and minimize costs. PCR-based methods are more sensitive and specific than conventional culture-based methods and provide rapid diagnostic results.
Mycoplasma
genitalium is a sexually transmitted infection. It is a common cause of
persistent urethritis. A polymerase chain reaction test can detect Mycoplasma
genitalium. Using this test, physicians can identify the presence of the
infection and provide the most accurate treatment possible.
Real-time
PCR technology has revolutionized Mycoplasma
Diagnostics testing for infections. It
is fast, inexpensive, and has high sensitivity. It also can identify the
organism without culture, making it a popular option in veterinary practice. Moreover,
PCR assays have been developed for four pathogenic avian mycoplasmas. The
earliest assays targeted the 16S rRNA gene, a region that is highly conserved.
This type of test has high sensitivity and cross-reactivity with other species
of mollicutes.
Fortunately,
there are two primary methods for detecting Mycoplasma hyopneumoniae in humans.
One method uses a fluorescent antibody that binds to the organism. The other is
an immunohistochemistry test that can detect the organism in fixed tissue. The
latter is often more practical for obtaining samples in the field, while the
former is more useful for detecting the disease in its early stages. Recently
in July 2020, bioMérieux, a globally leading in vitro diagnostics company
introduced BIO FIRE MYCOPLASMA, a new test for detection of mycoplasma in
pharmaceutical products that are used in biotherapeutics.
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